Guanidine isothiocyanate

The results indicated that all the RNA isolated by guanidine isothiocyanate method , SDS method and Tris-borated method were degraded and dispersed in some degrees .

结果表明，由异硫氰酸胍法、SDS法和Tris-硼酸法3种方法获得的RNA纯度较低，存在降解和弥散现象，或混杂的DNA、蛋白质较多，效果均不理想；

The total DNAs of fish oil , fish oil added with bovine DNA , fish oil added with goat DNA and butter were extracted by guanidine isothiocyanate method .

用异硫氰酸胍法提取鱼油、加入牛肉DNA的鱼油、加入山羊肉DNA的鱼油和牛油中的DNA。

Methods RNA was extracted by guanidine isothiocyanate method ;

方法采用异硫氢酸胍法提取RNA；

Methods Guanidine isothiocyanate was used to extract DNA and PCR-SSP was selected to type the HLA-DR genes .

方法采用异硫氰酸肌法抽提DNA，应用PCR－SSP方法进行HLA－DR分型。

DNAs were isolated from import feedstuff samples by guanidine isothiocyanate method . Bovine specific primers were synthesized and applied in the amplification and the length of target product was 271 bp .

该方法用异硫氰酸胍提取进口饲料样品DNA，以牛特异性引物扩增模板DNA，扩增产物为271bp，PCR产物经限制性酶切片段分析，与预期设想一致；